Kendra P. Rumbaugh, Iqbal Ahmad's Antibiofilm Agents: From Diagnosis to Treatment and PDF

By Kendra P. Rumbaugh, Iqbal Ahmad

ISBN-10: 3642538320

ISBN-13: 9783642538322

ISBN-10: 3642538339

ISBN-13: 9783642538339

This e-book presents a survey of modern advances within the improvement of antibiofilm brokers for scientific and environmental functions. the truth that microbes exist in established groups referred to as biofilms has slowly turn into approved in the clinical neighborhood. We now understand that over eighty% of all infectious ailments are biofilm-related; even if, major demanding situations nonetheless lie in our skill to diagnose and deal with those tremendous recalcitrant infections.

Written by means of specialists from world wide, this ebook deals a helpful source for doctors trying to deal with biofilm-related disorder, educational and researchers drawn to drug discovery and teachers who train classes on microbial pathogenesis and scientific microbiology.

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Example text

This led to the suspicion that the probe was nonspecifically binding to mast cells present in the tissue. This example serves to underscore the importance of appropriate controls and complementary alternative staining methods when using species-specific probes. A study published by Wallner and collaborators (1993) investigated the effect of several parameters on fluorescent rRNA-targeted oligonucleotide probe binding and specificity. These authors found that excessively high probe concentrations lead to an increase in nonspecific binding that was likely due to attachment of probes to cell components rather than mispairing of the probe sequence to nontarget sequences.

Bacteria in the tissue were clearly identifiable in the samples and ranged from micro-colonies to large clusters. A case report published in 2008 also reported the use of CLSM to investigate the cause of a persistent infection following a total joint arthroplasty (Stoodley et al. 2008). The patient had recurring pain and infection episodes for years despite several interventions. CSLM analysis of fluid, tissue, and cement collected at his final surgical revision revealed the presence of biofilm.

Pneumoniae by amoxicillin (Weimer et al. 2011). 24 R. B. Cox The clinical concern relative to the synergies of polymicrobial biofilm is that the infection will be more severe and recalcitrant to treatment. There are many examples which show that this is indeed the case. Low levels of P. aeruginosa mixed with Staphylococcus aureus increased infection rates in a rat model (Hendricks et al. 2001). In the mouse model, Prevotella increases the pathogenicity of S. aureus (Mikamo et al. 1998). Escherichia coli produced marked increase in the size of abscess formation with Bacteroides fragilis in a diabetic mouse model (Mastropaolo et al.

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Antibiofilm Agents: From Diagnosis to Treatment and Prevention by Kendra P. Rumbaugh, Iqbal Ahmad

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