By Robin D. Rogers, Andrew H. Bond, Cary B. Bauer (auth.), Robin D. Rogers, Mark A. Eiteman (eds.)
''A grand compilation...Well-bound, well-printed....It is certain that this pioneering booklet may help growing to be curiosity of the separation scientists in aqueous biphasic platforms and expand the scope of the field.'' --- Indian Chemical Society, 1998
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Extra resources for Aqueous Biphasic Separations: Biomolecules to Metal Ions
34. J. LOpez-Perez, Preparation of synaptosomes and mitochondria from manunalian brain, in: "Methods in Enzymology. Aqueous Two-Phase Systems," Vol. 228, pp. 403-411, H. Walter and G. , San Diego, CA (1994). 35. C. Larsson, Isolation of highly purified intact cWoroplasts and of multiorganelle complexes containing cWoroplasts, in: "Methods in Enzymology. Aqueous Two-Phase Systems," Vol. 228, pp. 419-424, H. Walter and G. , San Diego, CA (1994). 36. 1. Morre, 1. M. Morre, Plasma and internal membranes from cultured mammalian cells, in: "Methods in Enzymology.
We also know most ofthe factors which determine partition. Future research should be directed to find new phase systems with increased selectivity and polymers which allow an economical application on a technical scale. -A A1bertsson, Chromatography and partition ofcells and cel1 fragments, Nature 177:771 (1956). N. Bronsted, MolekUlgrosse und phasenverteilung. I, Z. Phys. , Abt. A Bodenstein-Festband:257 (1931). 3. 1. De Courdes, L. Rhumbler, Wilhelm Roux' Arch. Entwicklungsmech. Org. 7:225 (1898).
Such arguments lead to an equation of the form: 39 RTlnK = j (a+L\f) L\wz (2) Here, M is the solute's hydrophobicity, a value which may be related to the Gibbs free energy of transfer of the solute between the phases. The parameter j is a type of discrimination factor, indicating the difference in partition coefficients that is obtained by a unit change in solute hydrophobicity. The parameter a is related to that instrinsic hydrophobicity of the phase system, and will be merely referred to as the phase constant.
Aqueous Biphasic Separations: Biomolecules to Metal Ions by Robin D. Rogers, Andrew H. Bond, Cary B. Bauer (auth.), Robin D. Rogers, Mark A. Eiteman (eds.)